Egbono, F.F, Ogbonna Ugorji Nnaemeka, Obomanu-Tamunotonjo, R, Udeh, W.C and Edward Emmanuel Odinaka

Citation: Egbono, F.F, Ogbonna Ugorji Nnaemeka, Obomanu-Tamunotonjo, R, Udeh, W.C and Edward Emmanuel Odinaka (2025) Estimation of mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration effect of aqueous pulp extract of Raphia hookeri fruit in female Wistar rats. Frontline Professionals Journal, 2(1), 176–188
ABSTRACT
This research aimed at estimating mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration effect of aqueous pulp extract of Raphia Hookeri fruit in female wistar rats. A total of 28 female Wistar rats, weighing 130g to 200g, were grouped into 4 groups, Group 1 as control. The control group was provided standard feed and water, while Group 2 received 1000 mg/kg, Group 3 received 2000 mg/kg, Group 4 received 3000 mg/kg of APERHF daily for 28 days. After the treatment period, blood samples were collected, MCV, MCH, MCHC were analyzed using standard haematological techniques. Statistical analysis was conducted with SPSS version 21.0, results were expressed as SEM, with p<0.05 considered statistically significant. MCV levels increased significantly across treatment groups, with control at 72.00 ± 1.73, compared to 110.33 ± 2.60, 159.00 ± 2.31, and 125.00 ± 1.15 for Groups 2, 3, and 4, respectively. MCHC values decreased significantly in treated groups, with 412.00 ± 4.04 for control, and reductions to 272.00 ± 1.15, 191.00 ± 2.31, and 233.00 ± 1.73 for Groups 2, 3, and 4, respectively. These findings indicate that higher doses of APERHF may significantly impact parameters, suggesting potential haematological risks associated with prolonged high-dose administration.
KEYWORDS: Mean Corpuscular Volume, Haemoglobin Concentration